|
|
Registro completo
|
Biblioteca (s) : |
INIA La Estanzuela; INIA Tacuarembó. |
Fecha : |
21/02/2014 |
Actualizado : |
22/02/2014 |
Autor : |
Díaz, R.M. ; Baethgen, W.E. |
Título : |
Siete sistemas de rotación Agrícola-Ganaderos |
Fecha de publicación : |
1981 |
Fuente / Imprenta : |
ln: Seminario sobre sistemas en la Investigación Agropecuaria, 1981 : Set 14-18 : La Estanzuela.Colonia Colonia (Uruguay): IICA/BID, 1981. |
Páginas : |
p109-113 |
Idioma : |
Español |
Notas : |
Dentro del Programa Cooperativo de Investigación Agrícola en los países del Cono Sur |
Thesagro : |
ANALISIS DE COSTOS Y BENEFICIOS; ANALISIS ECONOMICO; DATOS DE PRODUCCION; PIENSOS; RENDIMIENTO DE CULTIVOS; ROTACION DE CULTIVOS; URUGUAY. |
Asunto categoría : |
-- |
Marc : |
LEADER 00795naa a2200229 a 4500 001 1045113 005 2014-02-22 008 1981 bl uuuu u00u1 u #d 100 1 $aDÍAZ, R.M. 245 $aSiete sistemas de rotación Agrícola-Ganaderos 260 $c1981 300 $ap109-113 500 $aDentro del Programa Cooperativo de Investigación Agrícola en los países del Cono Sur 650 $aANALISIS DE COSTOS Y BENEFICIOS 650 $aANALISIS ECONOMICO 650 $aDATOS DE PRODUCCION 650 $aPIENSOS 650 $aRENDIMIENTO DE CULTIVOS 650 $aROTACION DE CULTIVOS 650 $aURUGUAY 700 1 $aBAETHGEN, W.E. 773 $tln: Seminario sobre sistemas en la Investigación Agropecuaria, 1981 : Set 14-18 : La Estanzuela.Colonia Colonia (Uruguay): IICA/BID, 1981.
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA La Estanzuela (LE) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
Registro completo
|
Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
23/09/2019 |
Actualizado : |
23/09/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
B - 1 |
Autor : |
COUTO, D.; STRANSFELD, L.; ARRUABARRENA, A.; ZIPFEL, C.; LOZANO-DURÁN, R. |
Afiliación : |
DANIEL COUTO, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; LENA STRANSFELD, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CYRIL ZIPFEL, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; ROSA LOZANO-DURÁN, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; Shanghai Institutes of Biological Sciences, Shanghai Center for Plant Stress Biology (PSC), Chinese Academy of Sciences, China. |
Título : |
Broad application of a simple and affordable protocol for isolating plant RNA. (Short Report). |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
BMC Research Notes, 16 April 2015, Volume 8, Issue 1, Article number 154. OPEN ACCESS. |
ISSN : |
1756-0500 |
DOI : |
10.1186/s13104-015-1119-7 |
Idioma : |
Inglés |
Notas : |
Article history: Published on: 16 April 2015. |
Contenido : |
Abstract View references (4)
Background: Standard molecular biological methods involve the analysis of gene expression in living organisms under diverse environmental and developmental conditions. One of the most direct approaches to quantify gene expression is the isolation of RNA. Most techniques used to quantify gene expression require the isolation of RNA, usually from a large number of samples. While most published protocols, including those for commercial reagents, are either labour intensive, use hazardous chemicals and/or are costly, a previously published protocol for RNA isolation in Arabidopsis thaliana yields high amounts of good quality RNA in a simple, safe and inexpensive manner. Findings: We have tested this protocol in tomato and wheat leaves, as well as in Arabidopsis leaves, and compared the resulting RNA to that obtained using a commercial phenol-based reagent. Our results demonstrate that this protocol is applicable to other plant species, including monocots, and offers yield and purity at least comparable to those provided by commercial phenol-based reagents. Conclusions: Here, we show that this previously published RNA isolation protocol can be easily extended to other plant species without further modification. Due to its simplicity and the use of inexpensive reagents, this protocol is accessible and affordable and can be easily implemented to work on different plant species in laboratories worldwide.
© 2015 Couto et al.; licensee BioMed Central. |
Palabras claves : |
Affordable; Arabidopsis; Protocol; RNA; Tomato; Wheat. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13321/1/Couto-et-al.-2015.-BMC-Research-Notes.pdf
https://bmcresnotes.biomedcentral.com/track/pdf/10.1186/s13104-015-1119-7
|
Marc : |
LEADER 02324naa a2200277 a 4500 001 1060208 005 2019-09-23 008 2015 bl uuuu u00u1 u #d 022 $a1756-0500 024 7 $a10.1186/s13104-015-1119-7$2DOI 100 1 $aCOUTO, D. 245 $aBroad application of a simple and affordable protocol for isolating plant RNA. (Short Report).$h[electronic resource] 260 $c2015 500 $aArticle history: Published on: 16 April 2015. 520 $aAbstract View references (4) Background: Standard molecular biological methods involve the analysis of gene expression in living organisms under diverse environmental and developmental conditions. One of the most direct approaches to quantify gene expression is the isolation of RNA. Most techniques used to quantify gene expression require the isolation of RNA, usually from a large number of samples. While most published protocols, including those for commercial reagents, are either labour intensive, use hazardous chemicals and/or are costly, a previously published protocol for RNA isolation in Arabidopsis thaliana yields high amounts of good quality RNA in a simple, safe and inexpensive manner. Findings: We have tested this protocol in tomato and wheat leaves, as well as in Arabidopsis leaves, and compared the resulting RNA to that obtained using a commercial phenol-based reagent. Our results demonstrate that this protocol is applicable to other plant species, including monocots, and offers yield and purity at least comparable to those provided by commercial phenol-based reagents. Conclusions: Here, we show that this previously published RNA isolation protocol can be easily extended to other plant species without further modification. Due to its simplicity and the use of inexpensive reagents, this protocol is accessible and affordable and can be easily implemented to work on different plant species in laboratories worldwide. © 2015 Couto et al.; licensee BioMed Central. 653 $aAffordable 653 $aArabidopsis 653 $aProtocol 653 $aRNA 653 $aTomato 653 $aWheat 700 1 $aSTRANSFELD, L. 700 1 $aARRUABARRENA, A. 700 1 $aZIPFEL, C. 700 1 $aLOZANO-DURÁN, R. 773 $tBMC Research Notes, 16 April 2015, Volume 8, Issue 1, Article number 154. OPEN ACCESS.
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Las Brujas (LB) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
Expresión de búsqueda válido. Check! |
|
|